Here are the Advantages and disadvantages of HPLC and GC
| Catagory | HPLC | GC |
|---|---|---|
| Advantages | 1. Wide range of applications for both polar and nonpolar compounds. | 1. Excellent separation efficiency for volatile compounds. |
| 2. Suitable for analyzing thermally labile and high molecular weight compounds. | 2. Faster analysis time compared to HPLC. | |
| 3. Compatible with a wide variety of detectors. | 3. Lower detection limits and higher sensitivity. | |
| 4. Sample compatibility with aqueous and organic solvents. | 4. Ability to analyze gases, volatile liquids, and solids. | |
| 5. Can handle complex mixtures and provide good peak resolution. | 5. Greater choice of stationary phase options. | |
| 6. Lower cost per analysis due to reduced consumables. | ||
| Disadvantages | 1. Limited speed and throughput compared to GC. | 1. Limited applicability for nonvolatile compounds. |
| 2. Higher operational costs due to solvent usage. | 2. Thermal stability limitations for certain compounds. | |
| 3. Requires longer analysis time for separation. | 3. Limited column life and increased maintenance. | |
| 4. Relatively lower sensitivity compared to GC. | 4. Inability to analyze compounds with high boiling points. | |
| 5. Challenging for separating volatile compounds. | 5. Limited choice of detectors compared to HPLC. | |
| 6. More complex sample preparation requirements. |