Chromatography

Welcome to the Chromatography category! Here, you’ll find articles on one of the most widely used techniques in chemical analysis. Chromatography involves separating and identifying the components of a mixture based on their chemical and physical properties. From high-performance liquid chromatography (HPLC) to gas chromatography (GC), we’ll explore the different types of chromatography and how they are used in analytical chemistry. We’ll also discuss the latest trends and developments in chromatography and how it is being applied in various industries, including pharmaceuticals, food, and environmental monitoring. Join us as we dive into the world of chromatography and discover its many applications and benefits.

Flash Chromatography Decoded: Illuminating the Path to Purification Science

Chromatography / December 11, 2023

Flash chromatography, a powerful purification technique, stands as a beacon in the realm of chemical separation. As we delve into the intricate dance of molecules and stationary phases, this blog will illuminate the principles, techniques, and applications of flash chromatography. Join us on a journey through the world of chromatography, where speed meets precision, and …

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Theoretical plates in chromatography

Chromatography / December 11, 2023

Introduction Chromatography is a widely used technique in analytical chemistry for separating and analyzing components within a mixture. One crucial parameter that quantifies the separation efficiency in chromatography is the concept of theoretical plates. In this blog post, we will explore the meaning of theoretical plates, their significance in chromatographic separations, and how they are …

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Descending Chromatography

Chromatography / December 11, 2023

Introduction to Descending Chromatography Definition and Overview Descending chromatography, also known as descending paper chromatography, is a technique used to separate and analyze the components of a mixture. It belongs to the broader category of liquid chromatography, which involves the separation of compounds based on their differential interaction with a mobile phase and a stationary …

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Gas Chromatography Troubleshooting Guide

Chromatography / May 11, 2023

Introduction to Gas Chromatography Gas chromatography (GC) is a powerful analytical technique used for separating and analyzing volatile compounds. It involves the injection of a sample into a gas stream and the subsequent separation of the sample components using a chromatographic column. Despite its effectiveness, GC systems may encounter various issues that can affect the …

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Sample injector in HPLC

Chromatography / May 7, 2023

Introduction The separation of complicated mixtures into their constituent parts is accomplished using the commonly used analytical technique known as high-performance liquid chromatography (HPLC). The sample injector, which is in charge of putting the sample into the chromatographic column, is one of the most crucial parts of an HPLC system. Since even minor deviations in …

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Waters HPLC Column

Chromatography / April 30, 2023
Waters HPLC column

Introduction Brief overview of HPLC and its importance in various fields of research High-performance liquid chromatography (HPLC) is a powerful analytical technique used to separate, identify, and quantify the components of complex mixtures. It is widely used in various fields of research, including pharmaceuticals, environmental analysis, food and beverage analysis, forensic science, and more. HPLC …

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Mobile Phase in HPLC

Chromatography / December 11, 2023

Introduction High-performance liquid chromatography (HPLC) is a widely used technique in analytical chemistry, which is used to separate, identify, and quantify components of a mixture. The success of an HPLC separation largely depends on the properties of the mobile phase, which is the liquid solvent that flows through the stationary phase. The mobile phase should …

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UPLC: The High-Efficiency Analytical Technique Revolutionizing Chromatography

Chromatography / December 11, 2023

Introduction Ultra-Performance Liquid Chromatography (UPLC) is a powerful analytical technique used for the separation, identification, and quantification of complex mixtures. It is a high-resolution chromatographic technique that operates at high pressures, with sub-2 micron particle size columns, which provides better efficiency, resolution, sensitivity, and speed than conventional High-Performance Liquid Chromatography (HPLC). UPLC is a crucial …

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HPLC Detectors

Chromatography / December 11, 2023

Introduction High-performance liquid chromatography (HPLC) is a commonly used analytical technique that separates and quantifies chemical compounds in a liquid sample. The technique works by passing the sample through a stationary phase, where the components are separated based on their chemical properties. After separation, the individual components are detected and quantified by HPLC detectors. HPLC …

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What is HPLC? A Beginner’s Guide to High-Performance Liquid Chromatography

Chromatography / January 28, 2024

High-performance liquid chromatography, or HPLC, is a widely used analytical technique in chemistry and biochemistry. It is a powerful method for separating, identifying, and quantifying compounds in complex mixtures, making it an essential tool for a wide range of applications, from drug discovery and development to environmental analysis and food testing. In this beginner’s guide, …

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