Introduction Chromatography is a widely used technique in analytical chemistry for separating and analyzing components within a mixture. One crucial parameter that quantifies the separation efficiency in chromatography is the concept of theoretical plates. In this blog post, we will explore the meaning of theoretical plates, their significance in chromatographic separations, and how they are …
Introduction Potassium chloride (KCl) solutions serve as essential electrolytes in the field of pH measurement. pH meters, instruments utilized to determine the acidity or alkalinity of a solution, rely on stable and consistent reference electrolytes to provide accurate readings. By preparing a KCl solution specifically designed for pH meters, researchers can ensure reliable calibration and …
Introduction to Descending Chromatography Definition and Overview Descending chromatography, also known as descending paper chromatography, is a technique used to separate and analyze the components of a mixture. It belongs to the broader category of liquid chromatography, which involves the separation of compounds based on their differential interaction with a mobile phase and a stationary …
Introduction Measurement of temperature is essential in many different sectors and scientific fields. However, due to the inherent variability in temperature-sensitive equipment brought on by changes in ambient temperature, obtaining precise temperature measurements can be difficult. Automatic Temperature Compensation (ATC) is a method that engineers and scientists have created to get around this restriction. We …
Automatic Temperature Compensation: Enhancing Accuracy in Temperature Measurement Read More »
Here are the Advantages and disadvantages of HPLC and GC Catagory HPLC GC Advantages 1. Wide range of applications for both polar and nonpolar compounds. 1. Excellent separation efficiency for volatile compounds. 2. Suitable for analyzing thermally labile and high molecular weight compounds. 2. Faster analysis time compared to HPLC. 3. Compatible with a wide …
Introduction to Gas Chromatography Gas chromatography (GC) is a powerful analytical technique used for separating and analyzing volatile compounds. It involves the injection of a sample into a gas stream and the subsequent separation of the sample components using a chromatographic column. Despite its effectiveness, GC systems may encounter various issues that can affect the …
0.01n HCL preparation Remember to always consult the safety data sheet (SDS) and follow the specific guidelines provided by the manufacturer for handling, dilution, and storage of concentrated hydrochloric acid. If you are unsure or lack the necessary expertise, it is advisable to seek guidance from a qualified chemist or professional before attempting the preparation. …
How to Prepare 0.01n HCL solution and other concentrations of HCL Read More »
Here are some of the most Frequently asked Interview questions of HPLC. HPLC calibration Interview Questions HPLC troubleshooting interview questions HPLC CAlibration Interview Questions and Answers GC calibration Interview Questions
Introduction The separation of complicated mixtures into their constituent parts is accomplished using the commonly used analytical technique known as high-performance liquid chromatography (HPLC). The sample injector, which is in charge of putting the sample into the chromatographic column, is one of the most crucial parts of an HPLC system. Since even minor deviations in …
What are amines? Amines are a class of organic compounds that contain a nitrogen atom bonded to one or more alkyl or aromatic groups. They are derivatives of ammonia, where one or more of the hydrogen atoms have been replaced by alkyl or aromatic groups. Amines can be classified as primary, secondary, or tertiary, depending …
A Beginner’s Guide to Amines: What are they and Why are they Important? Read More »
