Difference between HPLC and TLC

Introduction High-performance liquid chromatography (HPLC) and thin-layer chromatography (TLC) are two commonly used chromatographic techniques in analytical chemistry. While both HPLC and TLC can be used to separate and identify chemical compounds, they differ in their principles, methods, and applications. In this blog post, we will explore the differences between HPLC and TLC. Difference Between …

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Waters HPLC Column

Waters HPLC column

Introduction Brief overview of HPLC and its importance in various fields of research High-performance liquid chromatography (HPLC) is a powerful analytical technique used to separate, identify, and quantify the components of complex mixtures. It is widely used in various fields of research, including pharmaceuticals, environmental analysis, food and beverage analysis, forensic science, and more. HPLC …

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Mobile Phase in HPLC

Introduction High-performance liquid chromatography (HPLC) is a widely used technique in analytical chemistry, which is used to separate, identify, and quantify components of a mixture. The success of an HPLC separation largely depends on the properties of the mobile phase, which is the liquid solvent that flows through the stationary phase. The mobile phase should …

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Accuracy and Precision in Analytical Chemistry: Understanding the Differences

Accuracy and precision are two important concepts in analytical chemistry that are often used interchangeably. However, they are distinct concepts that have different meanings and implications for analytical measurements. In this blog post, we will explore the differences between accuracy and precision, their importance in analytical chemistry, and how they can be evaluated. Accuracy in …

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UPLC: The High-Efficiency Analytical Technique Revolutionizing Chromatography

Introduction Ultra-Performance Liquid Chromatography (UPLC) is a powerful analytical technique used for the separation, identification, and quantification of complex mixtures. It is a high-resolution chromatographic technique that operates at high pressures, with sub-2 micron particle size columns, which provides better efficiency, resolution, sensitivity, and speed than conventional High-Performance Liquid Chromatography (HPLC). UPLC is a crucial …

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HPLC Vs UPLC

HPLC VS UPLC

Introduction When it comes to liquid chromatography, two of the most common techniques are high-performance liquid chromatography (HPLC) and ultra-high-performance liquid chromatography (UPLC). Both methods are used to separate and analyze complex mixtures, but they differ in several key ways. In this article, we’ll explore the differences between HPLC and UPLC and help you understand …

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HPLC Detectors

Introduction High-performance liquid chromatography (HPLC) is a commonly used analytical technique that separates and quantifies chemical compounds in a liquid sample. The technique works by passing the sample through a stationary phase, where the components are separated based on their chemical properties. After separation, the individual components are detected and quantified by HPLC detectors. HPLC …

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What is Molality?

What is Molality in Chemistry? Molality is a measure of the concentration of a solution, defined as the number of moles of solute per kilogram of solvent. It is often used in place of molarity, which describes the number of moles of solute per liter of solution, when the density of the solution varies with …

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What is Self Indicator?

What is Self Indicator? Self-indicators are substances that change color in response to changes in the acidity or basicity (pH) of a solution. These substances act as both the indicator and the analyte in a chemical reaction. This means that they are used to measure the pH of a solution without the need for an …

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Difference between UV and IR Spectroscopy

Difference Between UV and IR

Decoding the Distinctions: UV vs. IR Spectroscopy Introduction Spectroscopy is a powerful analytical technique that involves the study of the interaction between light and matter. It has found wide-ranging applications in various fields, including chemistry, physics, biochemistry, and materials science, among others. Two commonly used spectroscopic techniques are UV (Ultraviolet) and IR (Infrared) spectroscopy, which …

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